Selection and whole-genome sequencing of PZN-resistant mutants of B. anthracis membrane like other cell envelope-acting compounds but uniquely localizes to distinct foci within the envelope. anthracis, which revealed significant up-regulation of genes within the cell envelope stress response. We evaluate the underlying cause of this selective activity by measuring the RNA expression profile of PZN-treated B. anthracis from other members of the Bacillus cereus group. Here we report the remarkably discriminatory activity of PZN toward Bacillus anthracis, which rivals a previously described gamma (γ) phage lysis assay in distinguishing B. Extensive tailoring to 12 of the 14 amino acid residues in the mature natural product endows PZN with not only a rigid, polyheterocyclic structure, but also antibacterial activity. Plantazolicin (PZN) is a ribosomally synthesized and post-translationally modified natural product from Bacillus methylotrophicus FZB42 and Bacillus pumilus.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |